Background: Hypoxia is a hallmark of cancer and is associated with poor prognosis. However, the molecular mechanism by which hypoxia promotes tumor development is still unclear. MicroRNAs dysregulation has been shown to play an important role in the tumor and the tumor micro. Here, we investigated the role of miR-495 and miR-5688 in lung cancer of human non-small cell (NSCLC) and their underlying mechanisms.
Methods: The expression level of miR-495 and miR-5688 in human NSCLC tissue specimens was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Deferoxamine (DFO) is used to determine whether the regulation of miR-495 and miR-5688 under hypoxia depends on the hypoxia-inducible factor 1-alpha (HIF-1α).
Furthermore, the function of miR-495 and miR-5688 in tumor progression was evaluated using colony formation, 3- (4,5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H -tetrazolium (MTS), wound healing, transwell test, and the xenograft model. Two algorithms, PicTAR and Targetscan, used to predict the two miRNAs target gene is, and dual-luciferase reporter assay was performed to confirm the target. Tests are two-tailed paired t, Pearson correlation analysis and Fisher probability test conducted for statistical analysis.
Results: The two miRNAs, miR-495 and miR-5688, was found to participate in the development of NSCLC under hypoxia. They were down-regulated in NSCLC tissue compared to normal tissue. We determined that hypoxia causes down-regulation of miR-495 and miR-5688 in NSCLC cells, independent of HIF-1α and energy metabolism of cells. In addition, miR-495 and miR-5688 suppressed cell proliferation, migration and invasion in vitro
. The NSCLC xenograft models indicate that miR-495 and miR-5688 inhibits tumor formation in vivo. Interestingly, we found that miR-495 and miR-5688 have the same target, interleukin-11 (IL-11). Human recombinant IL-11 is counteracted the effects of miR-495 and miR-5688 in NSCLC cells, suggesting that miR-495 and miR-5688 held their tumor suppressor role by pressing IL-11 expression.
Conclusion: We found hypoxia down-regulated the expression level of miR-495 and miR-5688 in NSCLC to increase IL-11 expression and tumor progression, suggesting that miR-495 / miR-5688 / IL-11 axis can serve as a therapeutic target and a potential biomarker for NSCLC.
Variants in IL 6ST selectively IL – 11 signal defects in the Human and mouse Gp130 cytokine receptor subunit encoded by the IL 6ST is a cytokine receptors together for ten of IL -6 family. We describe non-identical homozygous variant in IL 6ST (p.R281Q) in patients with craniosynostosis and maintain primary teeth. We characterize the effects of variants in cytokine signaling in vitro using cell lines and cells transfected patient-derived primary and support these findings using mice models with appropriate genome -edited variant Il6st that p.R279Q.
Human Interleukin 17B (IL17B) Protein (Active) |
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abx651382-5mg | Abbexa | 5 mg | EUR 637.5 |
Recombinant Human Interleukin-17B protein(IL17B) (Active) |
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AP73458 | SAB | 1mg | EUR 3939 |
Recombinant Human Interleukin-17B protein (IL17B) (Active) |
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CSB-AP001651HU | Cusabio | 8251 mg | Ask for price |
Il17b (untagged) - Rat interleukin 17B (Il17b) |
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RN215972 | Origene Technologies GmbH | 10 µg | Ask for price |
IL17B (untagged)-Human interleukin 17B (IL17B) |
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SC304109 | Origene Technologies GmbH | 10 µg | Ask for price |
Interleukin 17B (IL17B) Antibody |
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20-abx101512 | Abbexa |
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Interleukin 17B (IL17B) Antibody |
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20-abx101513 | Abbexa |
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Interleukin 17B (IL17B) Antibody |
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abx038115-100ug | Abbexa | 100 ug | EUR 469.2 |
Interleukin 17B (IL17B) Antibody |
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abx234226-100ug | Abbexa | 100 ug | EUR 661.2 |
Interleukin 17B (IL17B) Antibody |
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20-abx173104 | Abbexa |
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Interleukin 17B (IL17B) Antibody |
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20-abx173105 | Abbexa |
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Interleukin 17B (IL17B) Antibody |
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20-abx216191 | Abbexa |
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Interleukin 17B (IL17B) Antibody |
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20-abx177117 | Abbexa |
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Interleukin 17B (IL17B) Antibody |
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20-abx177118 | Abbexa |
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Interleukin 17B (IL17B) Antibody |
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20-abx330118 | Abbexa |
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Interleukin 17B (IL17B) Antibody |
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abx101512-100l | Abbexa | 100 µl | EUR 262.5 |
Interleukin 17B (IL17B) Antibody |
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abx101512-1ml | Abbexa | 1 ml | EUR 712.5 |
Interleukin 17B (IL17B) Antibody |
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abx101512-200l | Abbexa | 200 µl | EUR 325 |
Interleukin 17B (IL17B) Antibody |
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abx101513-100l | Abbexa | 100 µl | EUR 275 |
Interleukin 17B (IL17B) Antibody |
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abx101513-1ml | Abbexa | 1 ml | EUR 775 |
Interleukin 17B (IL17B) Antibody |
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abx101513-200l | Abbexa | 200 µl | EUR 350 |
Interleukin 17B (IL17B) Antibody |
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abx173104-100l | Abbexa | 100 µl | EUR 750 |
Interleukin 17B (IL17B) Antibody |
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abx173104-1ml | Abbexa | 1 ml | Ask for price |
Interleukin 17B (IL17B) Antibody |
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abx173104-200l | Abbexa | 200 µl | Ask for price |
Interleukin 17B (IL17B) Antibody |
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abx173105-1ml | Abbexa | 1 ml | EUR 812.5 |
Interleukin 17B (IL17B) Antibody |
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abx177117-1096tests | Abbexa | 10 × 96 tests | EUR 725 |
Interleukin 17B (IL17B) Antibody |
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abx177117-596tests | Abbexa | 5 × 96 tests | EUR 337.5 |
Interleukin 17B (IL17B) Antibody |
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abx177117-96tests | Abbexa | 96 tests | EUR 262.5 |
Interleukin 17B (IL17B) Antibody |
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abx038115-500g | Abbexa | 500 µg | EUR 337.5 |
Interleukin 17B (IL17B) Antibody |
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abx330118-100g | Abbexa | 100 µg | Ask for price |
Interleukin 17B (IL17B) Antibody |
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abx330118-20g | Abbexa | 20 µg | EUR 187.5 |
Interleukin 17B (IL17B) Antibody |
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abx330118-50g | Abbexa | 50 µg | EUR 250 |
Interleukin 17B (IL17B) Antibody |
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abx375364-96tests | Abbexa | 96 tests | EUR 337.5 |
Recombinant Interleukin 17B (IL17B) |
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RPB700Hu01 | Cloud-Clone | 10ug | EUR 160 |
Recombinant Interleukin 17B (IL17B) |
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4-RPB700Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 17B expressed in: E.coli |
Recombinant Interleukin 17B (IL17B) |
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RPB700Mu01 | Cloud-Clone | 10ug | EUR 140 |
Recombinant Interleukin 17B (IL17B) |
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RPB700Ra01 | Cloud-Clone | 10ug | EUR 182 |
Recombinant Interleukin 17B (IL17B) |
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4-RPB700Ra01 | Cloud-Clone |
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Description: Recombinant Rat Interleukin 17B expressed in: E.coli |
Recombinant Interleukin 17B (IL17B) |
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RPU42250-100ug | Biomatik Corporation | 100ug | EUR 562.1 |
Recombinant Interleukin 17B (IL17B) |
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RPU42250-1mg | Biomatik Corporation | 1mg | EUR 2489.5 |
Recombinant Interleukin 17B (IL17B) |
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RPU42250-50ug | Biomatik Corporation | 50ug | EUR 451 |
Recombinant Interleukin 17B (IL17B) |
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RPU42251-100ug | Biomatik Corporation | 100ug | EUR 492.8 |
Recombinant Interleukin 17B (IL17B) |
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RPU42251-1mg | Biomatik Corporation | 1mg | EUR 2184 |
Recombinant Interleukin 17B (IL17B) |
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RPU42251-50ug | Biomatik Corporation | 50ug | EUR 396 |
Il17b (GFP-tagged) - Mouse interleukin 17B (Il17b) |
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MG201619 | Origene Technologies GmbH | 10 µg | Ask for price |
IL17B (GFP-tagged) - Human interleukin 17B (IL17B) |
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RG220051 | Origene Technologies GmbH | 10 µg | Ask for price |
Il17b (untagged) - Mouse interleukin 17B (Il17b), (10ug) |
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MC200167 | Origene Technologies GmbH | 10 µg | Ask for price |
Rat Interleukin 17B (IL17B) Protein |
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20-abx067473 | Abbexa |
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Rat Interleukin 17B (IL17B) Protein |
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abx067473-100g | Abbexa | 100 µg | EUR 587.5 |
Rat Interleukin 17B (IL17B) Protein |
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abx067473-10g | Abbexa | 10 µg | EUR 225 |
Rat Interleukin 17B (IL17B) Protein |
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abx067473-50g | Abbexa | 50 µg | EUR 412.5 |
Active Interleukin 12B (IL12B) |
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APA058Bo61 | Cloud-Clone | 10ug | EUR 380 |
Active Interleukin 12B (IL12B) |
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APA058Hu01 | Cloud-Clone | 10ug | EUR 358 |
Active Interleukin 28B (IL28B) |
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APC028Hu01 | Cloud-Clone | 10ug | EUR 211 |
Active Interleukin 28B (IL28B) |
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4-APC028Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 28B expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 28B (IL28B) |
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RPU55745-100ug | Biomatik Corporation | 100ug | EUR 651.2 |
Active Interleukin 28B (IL28B) |
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RPU55745-1mg | Biomatik Corporation | 1mg | EUR 3294.2 |
Active Interleukin 28B (IL28B) |
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RPU55745-50ug | Biomatik Corporation | 50ug | EUR 506.9 |
Active Interleukin 12B (IL12B) |
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RPU59255-100ug | Biomatik Corporation | 100ug | EUR 1183.1 |
Active Interleukin 12B (IL12B) |
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RPU59255-1mg | Biomatik Corporation | 1mg | EUR 5241.6 |
Active Interleukin 12B (IL12B) |
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RPU59255-50ug | Biomatik Corporation | 50ug | EUR 788.5 |
Human Interleukin 17B (IL17B) Protein |
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20-abx067475 | Abbexa |
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Mouse Interleukin 17B (IL17B) Protein |
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20-abx653985 | Abbexa |
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Mouse Interleukin 17B (IL17B) Protein |
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20-abx653986 | Abbexa |
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Human Interleukin 17B (IL17B) Protein |
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abx067475-100g | Abbexa | 100 µg | EUR 375 |
Human Interleukin 17B (IL17B) Protein |
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abx067475-200g | Abbexa | 200 µg | EUR 537.5 |
Human Interleukin 17B (IL17B) Protein |
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abx067475-50g | Abbexa | 50 µg | EUR 212.5 |
Human Interleukin 17B (IL17B) Protein |
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abx260754-10g | Abbexa | 10 µg | EUR 325 |
Human Interleukin 17B (IL17B) Protein |
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abx260754-2g | Abbexa | 2 µg | EUR 225 |
Human Interleukin 17B (IL17B) Protein |
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abx260760-10g | Abbexa | 10 µg | EUR 325 |
Human Interleukin 17B (IL17B) Protein |
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abx260760-2g | Abbexa | 2 µg | EUR 225 |
Mouse Interleukin 17B (IL17B) Protein |
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abx653985-100g | Abbexa | 100 µg | EUR 337.5 |
Mouse Interleukin 17B (IL17B) Protein |
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abx653985-200g | Abbexa | 200 µg | EUR 475 |
Mouse Interleukin 17B (IL17B) Protein |
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abx653985-50g | Abbexa | 50 µg | EUR 200 |
Il17b (myc-DDK-tagged) - Rat interleukin 17B (Il17b) |
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RR215972 | Origene Technologies GmbH | 10 µg | Ask for price |
Il17b (Myc-DDK-tagged) - Mouse interleukin 17B (Il17b) |
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MR201619 | Origene Technologies GmbH | 10 µg | Ask for price |
IL17B (Myc-DDK-tagged)-Human interleukin 17B (IL17B) |
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RC220051 | Origene Technologies GmbH | 10 µg | Ask for price |
Interleukin 17B (IL17B) Polyclonal Antibody |
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CAU25468-100ul | Biomatik Corporation | 100ul | EUR 242.9 |
Interleukin 17B (IL17B) Polyclonal Antibody |
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CAU25468-200ul | Biomatik Corporation | 200ul | EUR 304.1 |
Interleukin 17B (IL17B) Polyclonal Antibody |
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CAU25469-100ul | Biomatik Corporation | 100ul | EUR 222.7 |
Interleukin 17B (IL17B) Polyclonal Antibody |
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CAU25469-200ul | Biomatik Corporation | 200ul | EUR 278.9 |
Rat Interleukin 17B (IL17B) CLIA Kit |
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20-abx190699 | Abbexa |
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Rat Interleukin 17B (IL17B) CLIA Kit |
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EKN50810-48T | Biomatik Corporation | 48T | EUR 450.45 |
Rat Interleukin 17B (IL17B) CLIA Kit |
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EKN50810-5x96T | Biomatik Corporation | 5x96T | EUR 3056.63 |
Rat Interleukin 17B (IL17B) CLIA Kit |
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EKN50810-96T | Biomatik Corporation | 96T | EUR 643.5 |
Rat Interleukin 17B (IL17B) CLIA Kit |
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EKU09699-48T | Biomatik Corporation | 48T | EUR 724.22 |
Rat Interleukin 17B (IL17B) CLIA Kit |
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EKU09699-5x96T | Biomatik Corporation | 5x96T | EUR 4914.35 |
Rat Interleukin 17B (IL17B) CLIA Kit |
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EKU09699-96T | Biomatik Corporation | 96T | EUR 1034.6 |
CLIA Kit for Interleukin 17B (IL17B) |
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SCB700Hu | Cloud-Clone | 96Т | EUR 840 |
CLIA Kit for Interleukin 17B (IL17B) |
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SCB700Mu | Cloud-Clone | 96Т | EUR 864 |
CLIA Kit for Interleukin 17B (IL17B) |
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SCB700Ra | Cloud-Clone | 96Т | EUR 912 |
Rat Interleukin 17B (IL17B)CLIA Kit |
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SCB700Ra-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 7342.85 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Interleukin 17B (IL17B) in serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B)CLIA Kit |
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SCB700Ra-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 709.88 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Interleukin 17B (IL17B) in serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B)CLIA Kit |
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SCB700Ra-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 962.69 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Interleukin 17B (IL17B) in serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B)CLIA Kit |
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SCB700Ra-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3972.1 |
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Interleukin 17B (IL17B) in serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B) CLIA Kit |
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4-SCB700Ra | Cloud-Clone |
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Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Rat Interleukin 17B (IL17B)Serum, plasma and other biological fluids |
Rat Interleukin 17B (IL17B) ELISA Kit |
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20-abx155721 | Abbexa |
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Rat Interleukin 17B (IL17B) ELISA Kit |
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DLR-IL17B-Ra | DL Develop | 96T | EUR 417 |
Description: serum, plasma or other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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DLR-IL17B-Ra-48T | DL Develop | 48T | EUR 570 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 17B (IL17B) in samples from serum, plasma or other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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DLR-IL17B-Ra-96T | DL Develop | 96T | EUR 739.2 |
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 17B (IL17B) in samples from serum, plasma or other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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EKN46354-48T | Biomatik Corporation | 48T | EUR 390.46 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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EKN46354-5x96T | Biomatik Corporation | 5x96T | EUR 2649.55 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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EKN46354-96T | Biomatik Corporation | 96T | EUR 557.8 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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EKU05243-48T | Biomatik Corporation | 48T | EUR 573.02 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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EKU05243-5x96T | Biomatik Corporation | 5x96T | EUR 3888.35 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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EKU05243-96T | Biomatik Corporation | 96T | EUR 818.6 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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DL-IL17B-Ra | DL Develop | 96T | EUR 397 |
Description: serum, plasma or other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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EK14780 | SAB | 96Т | EUR 768 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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RDR-IL17B-Ra-48T | Reddot Biotech | 48T | EUR 427.56 |
Description: serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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RDR-IL17B-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 594 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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RDR-IL17B-Ra-96T | Reddot Biotech | 96T | EUR 610.79 |
Description: serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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RDR-IL17B-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 822 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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RD-IL17B-Ra-48T | Reddot Biotech | 48T | EUR 407.2 |
Description: serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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RD-IL17B-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 567.6 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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RD-IL17B-Ra-96T | Reddot Biotech | 96T | EUR 581.7 |
Description: serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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RD-IL17B-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 786 |
ELISA Kit for Interleukin 17B (IL17B) |
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SEB700Hu | Cloud-Clone | 96Т | EUR 665 |
ELISA Kit for Interleukin 17B (IL17B) |
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SEB700Mu | Cloud-Clone | 96Т | EUR 684 |
ELISA Kit for Interleukin 17B (IL17B) |
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SEB700Ra | Cloud-Clone | 96Т | EUR 722 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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SEB700Ra-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5850.59 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 17B (IL17B) in serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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SEB700Ra-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 586.99 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 17B (IL17B) in serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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SEB700Ra-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 787.13 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 17B (IL17B) in serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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SEB700Ra-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 3182.08 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 17B (IL17B) in serum, plasma and other biological fluids. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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4-SEB700Ra | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Interleukin 17B (IL17B) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species. |
Rat Interleukin 17B (IL17B) ELISA Kit |
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abx155721-100g | Abbexa | 100 µg | EUR 687.5 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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abx155721-1mg | Abbexa | 1 mg | EUR 6187.5 |
Rat Interleukin 17B (IL17B) ELISA Kit |
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abx155721-200g | Abbexa | 200 µg | EUR 3275 |
Human Interleukin 17B (IL17B) CLIA Kit |
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20-abx190244 | Abbexa |
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Mouse Interleukin 17B (IL17B) CLIA Kit |
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20-abx190529 | Abbexa |
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We showed that Human p.R281Q gp130 associated with selective loss IL – 11 signals without affecting the IL – 6 IL -27, OSM, LIF, CT1, CLC, and CNTF signal. In mice Il6st p.R279Q reduce the size of garbage and cause synostosis facial and dental abnormalities. Effects on IL – 11 signaling induced by gp130 variants showed incomplete penetrance but phenocopies aspect IL 11 RA deficiencies in the human and mice. Our data indicate that genetically in pleiotropic cytokine receptor variants may have a very selective flaw.