Introduction CRISPR / system CAS9, which can be utilized in vitro biological experiments have recently caught a lot of attention for their important role and benefits. However, realization of the full potential of CRISPR / CAS9 approach requires addressing the various challenges and side effects. Expression gene and the potential side effects of the CRISPR / CAS9 in Human cells that are still to be explained.
The aim of our study was to explore the effect of CRISPR / CAS9 on gene expression in 293T cells. A Cas9-expressing plasmid PX458 and PX458 Cas9-T2A-off plasmids used to study the role of CRISPR / CAS9 at set gene expression in 293T cells. Gene expression in 293T cells after transfection of plasmid PX458 or PX458-T2A plasmid RNA was detected by sequencing and correlative statistical analysis. Differential gene expression in transfected 293T second PX458 and PX458-T2A cells transfected 293T 293T cells compared with normal cells detected using chain quantitative reverse transcription polymerase reaction (RT qPCR). MRNA and protein levels were measured using reverse transcription PCR and Western blot.
Co-IP assay combined with a shotgun LC-MS / MS is used to investigate differences NGFR protein-interaction between PX458 and PX458 transfected 293T cells transfected 293T cell-T2A. In this study, we observed that the PX458 plasmid transfection and expression Cas9 can affect the expression of different gene , including FOSB (FBJ murine osteosarcoma virus Onco gene homolog B), IL – 11 (Interleukin- 11 ), MMP1 (matrix metalloproteinases), CYP2D6 (CytochromeP4502D6), and NGFR (matrix metalloproteinase 1).

Downregulation of NGFR after transfection PX458 confirmed by RT qPCR and western blot analysis. NGFR expression was significantly lowered in PX458 transfected 293T 293T cells than in normal cells and PX458-T2A transfected 293T cells. Dilution co-IP was analyzed with a shotgun LC-MS / MS showed total 183 proteins interact with NGFR in 293T cells transfected PX458 while 221 proteins interact with NGFR-T2A PX458 identified in transfected 293T cells using machine mascot.
Cas9 expression by transfection of plasmid PX458 negatively correlated with the level NGFR NGFR mRNA and protein expression in 293T cells, while the PX458-T2A, where Cas9 disabled, does not affect the expression of NGFR. Decreased expression of NGFR also affects the number of proteins that interact with NGFR. These results indicate that the effect Cas9 in NGFR expression and other expressions gene should be considered when developing cell-based research and therapy utilizing the CRISPR / CAS9 system.
Profile inflammation and osteogenic hematoma Fracture Human Potential. form of fracture hematoma immediately after the fracture and is considered important for bone healing process. molecular composition has been briefly investigated the existence of our current understanding is based on animal studies.
Active Interleukin 13 (IL13) |
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RPU56351-100ug | Biomatik Corporation | 100ug | EUR 985.6 |
Active Interleukin 13 (IL13) |
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RPU56351-1mg | Biomatik Corporation | 1mg | EUR 4941.3 |
Active Interleukin 13 (IL13) |
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RPU56351-50ug | Biomatik Corporation | 50ug | EUR 760.4 |
Human Interleukin 13 (IL13) Protein (Active) |
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20-abx655715 | Abbexa |
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Active Interleukin 10 (IL10) |
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4-APA056Hu61 | Cloud-Clone |
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Description: Recombinant Human Interleukin 10 expressed in: 293F cell |
Active Interleukin 11 (IL11) |
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4-APA057Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 11 expressed in: E.coli |
Active Interleukin 15 (IL15) |
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4-APA061Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 15 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 16 (IL16) |
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4-APA062Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 16 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 17 (IL17) |
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4-APA063Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 17 expressed in: E.coli |
Active Interleukin 18 (IL18) |
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4-APA064Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 18 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 18 (IL18) |
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4-APA064Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Interleukin 18 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 21 (IL21) |
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4-APB688Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 21 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 25 (IL25) |
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4-APB694Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 25 expressed in: E.coli |
Active Interleukin 33 (IL33) |
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4-APB980Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 33 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 34 (IL34) |
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4-APC007Hu01 | Cloud-Clone |
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Description: Recombinant Human Active Interleukin 34 (IL34) expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 35 (IL35) |
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4-APC008Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 35 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 29 (IL29) |
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4-APC029Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 29 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 22 (IL22) |
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4-APC032Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 22 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 20 (IL20) |
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4-APC058Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 20 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 24 (IL24) |
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4-APC064Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 24 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 10 (IL10) |
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RPU54838-100ug | Biomatik Corporation | 100ug | EUR 1030.7 |
Active Interleukin 10 (IL10) |
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RPU54838-1mg | Biomatik Corporation | 1mg | EUR 4569.5 |
Active Interleukin 10 (IL10) |
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RPU54838-50ug | Biomatik Corporation | 50ug | EUR 828.3 |
Active Interleukin 11 (IL11) |
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RPU54855-100ug | Biomatik Corporation | 100ug | EUR 1108.8 |
Active Interleukin 11 (IL11) |
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RPU54855-1mg | Biomatik Corporation | 1mg | EUR 4914 |
Active Interleukin 11 (IL11) |
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RPU54855-50ug | Biomatik Corporation | 50ug | EUR 891 |
Active Interleukin 15 (IL15) |
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RPU54856-100ug | Biomatik Corporation | 100ug | EUR 1232 |
Active Interleukin 15 (IL15) |
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RPU54856-1mg | Biomatik Corporation | 1mg | EUR 6240 |
Active Interleukin 15 (IL15) |
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RPU54856-50ug | Biomatik Corporation | 50ug | EUR 990 |
Active Interleukin 16 (IL16) |
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RPU54857-100ug | Biomatik Corporation | 100ug | EUR 1108.8 |
Active Interleukin 16 (IL16) |
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RPU54857-1mg | Biomatik Corporation | 1mg | EUR 4914 |
Active Interleukin 16 (IL16) |
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RPU54857-50ug | Biomatik Corporation | 50ug | EUR 891 |
Active Interleukin 17 (IL17) |
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RPU54858-100ug | Biomatik Corporation | 100ug | EUR 514.8 |
Active Interleukin 17 (IL17) |
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RPU54858-1mg | Biomatik Corporation | 1mg | EUR 2607.8 |
Active Interleukin 17 (IL17) |
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RPU54858-50ug | Biomatik Corporation | 50ug | EUR 401.3 |
Active Interleukin 18 (IL18) |
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RPU54859-100ug | Biomatik Corporation | 100ug | EUR 722.7 |
Active Interleukin 18 (IL18) |
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RPU54859-1mg | Biomatik Corporation | 1mg | EUR 3199.3 |
Active Interleukin 18 (IL18) |
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RPU54859-50ug | Biomatik Corporation | 50ug | EUR 579.7 |
Active Interleukin 25 (IL25) |
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RPU54937-100ug | Biomatik Corporation | 100ug | EUR 739.2 |
Active Interleukin 25 (IL25) |
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RPU54937-1mg | Biomatik Corporation | 1mg | EUR 3276 |
Active Interleukin 25 (IL25) |
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RPU54937-50ug | Biomatik Corporation | 50ug | EUR 594 |
Active Interleukin 35 (IL35) |
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RPU54947-100ug | Biomatik Corporation | 100ug | EUR 616 |
Active Interleukin 35 (IL35) |
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RPU54947-1mg | Biomatik Corporation | 1mg | EUR 2730 |
Active Interleukin 35 (IL35) |
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RPU54947-50ug | Biomatik Corporation | 50ug | EUR 495 |
Active Interleukin 20 (IL20) |
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RPU54948-100ug | Biomatik Corporation | 100ug | EUR 675.4 |
Active Interleukin 20 (IL20) |
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RPU54948-1mg | Biomatik Corporation | 1mg | EUR 3419 |
Active Interleukin 20 (IL20) |
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RPU54948-50ug | Biomatik Corporation | 50ug | EUR 526.1 |
Active Interleukin 24 (IL24) |
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RPU54949-100ug | Biomatik Corporation | 100ug | EUR 651.2 |
Active Interleukin 24 (IL24) |
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RPU54949-1mg | Biomatik Corporation | 1mg | EUR 3294.2 |
Active Interleukin 24 (IL24) |
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RPU54949-50ug | Biomatik Corporation | 50ug | EUR 506.9 |
Active Interleukin 18 (IL18) |
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RPU55467-100ug | Biomatik Corporation | 100ug | EUR 739.2 |
Active Interleukin 18 (IL18) |
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RPU55467-1mg | Biomatik Corporation | 1mg | EUR 3731 |
Active Interleukin 18 (IL18) |
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RPU55467-50ug | Biomatik Corporation | 50ug | EUR 574.1 |
Active Interleukin 21 (IL21) |
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RPU55704-100ug | Biomatik Corporation | 100ug | EUR 997.7 |
Active Interleukin 21 (IL21) |
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RPU55704-1mg | Biomatik Corporation | 1mg | EUR 3893.5 |
Active Interleukin 21 (IL21) |
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RPU55704-50ug | Biomatik Corporation | 50ug | EUR 599.1 |
Active Interleukin 33 (IL33) |
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RPU55739-100ug | Biomatik Corporation | 100ug | EUR 1013.1 |
Active Interleukin 33 (IL33) |
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RPU55739-1mg | Biomatik Corporation | 1mg | EUR 5116.8 |
Active Interleukin 33 (IL33) |
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RPU55739-50ug | Biomatik Corporation | 50ug | EUR 787.2 |
Active Interleukin 34 (IL34) |
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RPU55743-100ug | Biomatik Corporation | 100ug | EUR 997.7 |
Active Interleukin 34 (IL34) |
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RPU55743-1mg | Biomatik Corporation | 1mg | EUR 3931.2 |
Active Interleukin 34 (IL34) |
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RPU55743-50ug | Biomatik Corporation | 50ug | EUR 604.8 |
Active Interleukin 29 (IL29) |
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RPU55746-100ug | Biomatik Corporation | 100ug | EUR 651.2 |
Active Interleukin 29 (IL29) |
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RPU55746-1mg | Biomatik Corporation | 1mg | EUR 3294.2 |
Active Interleukin 29 (IL29) |
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RPU55746-50ug | Biomatik Corporation | 50ug | EUR 506.9 |
Active Interleukin 22 (IL22) |
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RPU55748-100ug | Biomatik Corporation | 100ug | EUR 1282.6 |
Active Interleukin 22 (IL22) |
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RPU55748-1mg | Biomatik Corporation | 1mg | EUR 5054.4 |
Active Interleukin 22 (IL22) |
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RPU55748-50ug | Biomatik Corporation | 50ug | EUR 777.6 |
Active Interleukin 32 (IL32) |
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RPU58896-100ug | Biomatik Corporation | 100ug | EUR 709.8 |
Active Interleukin 32 (IL32) |
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RPU58896-1mg | Biomatik Corporation | 1mg | EUR 3294.7 |
Active Interleukin 32 (IL32) |
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RPU58896-50ug | Biomatik Corporation | 50ug | EUR 522.7 |
Active Interleukin 19 (IL19) |
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RPU58930-100ug | Biomatik Corporation | 100ug | EUR 868.2 |
Active Interleukin 19 (IL19) |
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RPU58930-1mg | Biomatik Corporation | 1mg | EUR 3419.5 |
Active Interleukin 19 (IL19) |
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RPU58930-50ug | Biomatik Corporation | 50ug | EUR 542.5 |
Active Interleukin 10 (IL10) |
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RPU59057-100ug | Biomatik Corporation | 100ug | EUR 939.7 |
Active Interleukin 10 (IL10) |
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RPU59057-1mg | Biomatik Corporation | 1mg | EUR 3673.8 |
Active Interleukin 10 (IL10) |
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RPU59057-50ug | Biomatik Corporation | 50ug | EUR 587.2 |
Active Interleukin 31 (IL31) |
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RPU59177-100ug | Biomatik Corporation | 100ug | EUR 1084.2 |
Active Interleukin 31 (IL31) |
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RPU59177-1mg | Biomatik Corporation | 1mg | EUR 4504.5 |
Active Interleukin 31 (IL31) |
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RPU59177-50ug | Biomatik Corporation | 50ug | EUR 720 |
Active Interleukin 22 (IL22) |
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RPU59213-100ug | Biomatik Corporation | 100ug | EUR 1069.2 |
Active Interleukin 22 (IL22) |
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RPU59213-1mg | Biomatik Corporation | 1mg | EUR 4738.5 |
Active Interleukin 22 (IL22) |
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RPU59213-50ug | Biomatik Corporation | 50ug | EUR 712.8 |
Active Interleukin 10 (IL10) |
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RPU59216-100ug | Biomatik Corporation | 100ug | EUR 1082.4 |
Active Interleukin 10 (IL10) |
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RPU59216-1mg | Biomatik Corporation | 1mg | EUR 4797 |
Active Interleukin 10 (IL10) |
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RPU59216-50ug | Biomatik Corporation | 50ug | EUR 721.6 |
Active Interleukin 11 (IL11) |
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RPU59219-100ug | Biomatik Corporation | 100ug | EUR 1084.1 |
Active Interleukin 11 (IL11) |
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RPU59219-1mg | Biomatik Corporation | 1mg | EUR 4802.9 |
Active Interleukin 11 (IL11) |
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RPU59219-50ug | Biomatik Corporation | 50ug | EUR 722.5 |
Active Interleukin 26 (IL26) |
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RPU59230-100ug | Biomatik Corporation | 100ug | EUR 1093.1 |
Active Interleukin 26 (IL26) |
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RPU59230-1mg | Biomatik Corporation | 1mg | EUR 4843.8 |
Active Interleukin 26 (IL26) |
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RPU59230-50ug | Biomatik Corporation | 50ug | EUR 728.6 |
Active Interleukin 10 (IL10) |
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RPU56353-100ug | Biomatik Corporation | 100ug | EUR 1282.6 |
Active Interleukin 10 (IL10) |
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RPU56353-1mg | Biomatik Corporation | 1mg | EUR 5054.4 |
Active Interleukin 10 (IL10) |
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RPU56353-50ug | Biomatik Corporation | 50ug | EUR 777.6 |
Active Interleukin 10 (IL10) |
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RPU56368-100ug | Biomatik Corporation | 100ug | EUR 1078 |
Active Interleukin 10 (IL10) |
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RPU56368-1mg | Biomatik Corporation | 1mg | EUR 4777.5 |
Active Interleukin 10 (IL10) |
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RPU56368-50ug | Biomatik Corporation | 50ug | EUR 866.8 |
Active Interleukin 2 (IL2) |
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4-APA073Hu61 | Cloud-Clone |
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Description: Recombinant Human Interleukin 2 expressed in: 293F cell |
Active Interleukin 2 (IL2) |
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4-APA073Mu61 | Cloud-Clone |
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Description: Recombinant Mouse Interleukin 2 expressed in: 293F cell |
Active Interleukin 4 (IL4) |
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4-APA077Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 4 expressed in: E.coli |
Active Interleukin 4 (IL4) |
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4-APA077Hu61 | Cloud-Clone |
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Description: Recombinant Human Interleukin 4 expressed in: 293F cell |
Active Interleukin 4 (IL4) |
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4-APA077Mu01 | Cloud-Clone |
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Description: Recombinant Mouse Interleukin 4 expressed in: E.coli |
Active Interleukin 4 (IL4) |
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4-APA077Ra01 | Cloud-Clone |
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Description: Recombinant Rat Interleukin 4 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 6 (IL6) |
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4-APA079Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 6 expressed in: E.coli |
Active Interleukin 6 (IL6) |
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4-APA079Hu61 | Cloud-Clone |
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Description: Recombinant Human Interleukin 6 expressed in: 293F cell |
Active Interleukin 6 (IL6) |
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4-APA079Mu61 | Cloud-Clone |
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Description: Recombinant Mouse Interleukin 6 expressed in: 293F cell |
Active Interleukin 6 (IL6) |
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4-APA079Ra61 | Cloud-Clone |
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Description: Recombinant Rat Interleukin 6 expressed in: 293F cell |
Active Interleukin 8 (IL8) |
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4-APA080Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 8 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 7 (IL7) |
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4-APA662Hu01 | Cloud-Clone |
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Description: Recombinant Human Interleukin 7 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells |
Active Interleukin 4 (IL4) |
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RPU54749-100ug | Biomatik Corporation | 100ug | EUR 777.7 |
Active Interleukin 4 (IL4) |
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RPU54749-1mg | Biomatik Corporation | 1mg | EUR 3445 |
Active Interleukin 4 (IL4) |
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RPU54749-50ug | Biomatik Corporation | 50ug | EUR 624.8 |
Active Interleukin 2 (IL2) |
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RPU54751-100ug | Biomatik Corporation | 100ug | EUR 453.2 |
Active Interleukin 2 (IL2) |
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RPU54751-1mg | Biomatik Corporation | 1mg | EUR 2009.8 |
Active Interleukin 2 (IL2) |
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RPU54751-50ug | Biomatik Corporation | 50ug | EUR 364.1 |
Active Interleukin 4 (IL4) |
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RPU54828-100ug | Biomatik Corporation | 100ug | EUR 822.8 |
Active Interleukin 4 (IL4) |
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RPU54828-1mg | Biomatik Corporation | 1mg | EUR 3244.8 |
Active Interleukin 4 (IL4) |
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RPU54828-50ug | Biomatik Corporation | 50ug | EUR 499.2 |
Active Interleukin 6 (IL6) |
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RPU54829-100ug | Biomatik Corporation | 100ug | EUR 1045 |
Active Interleukin 6 (IL6) |
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RPU54829-1mg | Biomatik Corporation | 1mg | EUR 5253.3 |
Active Interleukin 6 (IL6) |
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RPU54829-50ug | Biomatik Corporation | 50ug | EUR 808.4 |
Active Interleukin 6 (IL6) |
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RPU54832-100ug | Biomatik Corporation | 100ug | EUR 971.3 |
The purpose of this study to analyze the content of inflammatory cytokines in the fracture hematoma Human and determine their effect on osteoprogenitor cells. Twenty-three patients were recruited following informed consent. peripheral blood, and bone fracture hematoma collected. A Luminex assay at the level of 34 cytokines done and autologous peripheral blood samples served as controls. Mesenchymal Stem Cells (MSCs) were isolated following collagenase digestion and functional tests performed. Gene expression analysis of 84 key osteogenic molecules do.