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Cas9 Protein Triggers Differential Expression of Inherent Genes Especially NGFR Expression in 293T Cells.

Cas9 Protein Triggers Differential Expression of Inherent Genes Especially NGFR Expression in 293T Cells.
Posted by Rachael

Introduction CRISPR / system CAS9, which can be utilized in vitro biological experiments have recently caught a lot of attention for their important role and benefits. However, realization of the full potential of CRISPR / CAS9 approach requires addressing the various challenges and side effects. Expression gene and the potential side effects of the CRISPR / CAS9 in Human cells that are still to be explained.

The aim of our study was to explore the effect of CRISPR / CAS9 on gene expression in 293T cells. A Cas9-expressing plasmid PX458 and PX458 Cas9-T2A-off plasmids used to study the role of CRISPR / CAS9 at set gene expression in 293T cells. Gene expression in 293T cells after transfection of plasmid PX458 or PX458-T2A plasmid RNA was detected by sequencing and correlative statistical analysis. Differential gene expression in transfected 293T second PX458 and PX458-T2A cells transfected 293T 293T cells compared with normal cells detected using chain quantitative reverse transcription polymerase reaction (RT qPCR). MRNA and protein levels were measured using reverse transcription PCR and Western blot.

Co-IP assay combined with a shotgun LC-MS / MS is used to investigate differences NGFR protein-interaction between PX458 and PX458 transfected 293T cells transfected 293T cell-T2A. In this study, we observed that the PX458 plasmid transfection and expression Cas9 can affect the expression of different gene , including FOSB (FBJ murine osteosarcoma virus Onco gene homolog B), IL – 11 (Interleukin- 11 ), MMP1 (matrix metalloproteinases), CYP2D6 (CytochromeP4502D6), and NGFR (matrix metalloproteinase 1).

Cas9 Protein Triggers Differential Expression of Inherent Genes Especially NGFR Expression in 293T Cells.
Cas9 Protein Triggers Differential Expression of Inherent Genes Especially NGFR Expression in 293T Cells.

Downregulation of NGFR after transfection PX458 confirmed by RT qPCR and western blot analysis. NGFR expression was significantly lowered in PX458 transfected 293T 293T cells than in normal cells and PX458-T2A transfected 293T cells. Dilution co-IP was analyzed with a shotgun LC-MS / MS showed total 183 proteins interact with NGFR in 293T cells transfected PX458 while 221 proteins interact with NGFR-T2A PX458 identified in transfected 293T cells using machine mascot.

Cas9 expression by transfection of plasmid PX458 negatively correlated with the level NGFR NGFR mRNA and protein expression in 293T cells, while the PX458-T2A, where Cas9 disabled, does not affect the expression of NGFR. Decreased expression of NGFR also affects the number of proteins that interact with NGFR. These results indicate that the effect Cas9 in NGFR expression and other expressions gene should be considered when developing cell-based research and therapy utilizing the CRISPR / CAS9 system.

Profile inflammation and osteogenic hematoma Fracture Human Potential. form of fracture hematoma immediately after the fracture and is considered important for bone healing process. molecular composition has been briefly investigated the existence of our current understanding is based on animal studies.

Active Interleukin 13 (IL13)

RPU56351-100ug 100ug
EUR 985.6

Active Interleukin 13 (IL13)

RPU56351-1mg 1mg
EUR 4941.3

Active Interleukin 13 (IL13)

RPU56351-50ug 50ug
EUR 760.4

Human Interleukin 13 (IL13) Protein (Active)

20-abx655715
  • EUR 1328.40
  • EUR 477.60
  • EUR 4436.40
  • EUR 1612.80
  • EUR 910.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Active Interleukin 10 (IL10)

4-APA056Hu61
  • EUR 1019.94
  • EUR 397.20
  • EUR 3494.78
  • EUR 1244.93
  • EUR 2369.86
  • EUR 758.40
  • EUR 8556.96
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 10 expressed in: 293F cell

Active Interleukin 11 (IL11)

4-APA057Hu01
  • EUR 1087.68
  • EUR 415.20
  • EUR 3748.80
  • EUR 1329.60
  • EUR 2539.20
  • EUR 804.00
  • EUR 9192.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 11 expressed in: E.coli

Active Interleukin 15 (IL15)

4-APA061Hu01
  • EUR 1195.20
  • EUR 444.00
  • EUR 4152.00
  • EUR 1464.00
  • EUR 2808.00
  • EUR 876.00
  • EUR 10200.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 15 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 16 (IL16)

4-APA062Hu01
  • EUR 1087.68
  • EUR 415.20
  • EUR 3748.80
  • EUR 1329.60
  • EUR 2539.20
  • EUR 804.00
  • EUR 9192.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 16 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 17 (IL17)

4-APA063Hu01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 17 expressed in: E.coli

Active Interleukin 18 (IL18)

4-APA064Hu01
  • EUR 797.38
  • EUR 337.20
  • EUR 2660.16
  • EUR 966.72
  • EUR 1813.44
  • EUR 609.60
  • EUR 6470.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 18 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 18 (IL18)

4-APA064Mu01
  • EUR 689.86
  • EUR 308.40
  • EUR 2256.96
  • EUR 832.32
  • EUR 1544.64
  • EUR 537.60
  • EUR 5462.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Interleukin 18 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 21 (IL21)

4-APB688Hu01
  • EUR 790.92
  • EUR 334.80
  • EUR 2635.97
  • EUR 958.66
  • EUR 1797.31
  • EUR 604.80
  • EUR 6409.92
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 21 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 25 (IL25)

4-APB694Hu01
  • EUR 765.12
  • EUR 328.80
  • EUR 2539.20
  • EUR 926.40
  • EUR 1732.80
  • EUR 588.00
  • EUR 6168.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 25 expressed in: E.coli

Active Interleukin 33 (IL33)

4-APB980Hu01
  • EUR 1060.80
  • EUR 408.00
  • EUR 3648.00
  • EUR 1296.00
  • EUR 2472.00
  • EUR 786.00
  • EUR 8940.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 33 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 34 (IL34)

4-APC007Hu01
  • EUR 797.38
  • EUR 337.20
  • EUR 2660.16
  • EUR 966.72
  • EUR 1813.44
  • EUR 609.60
  • EUR 6470.40
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Active Interleukin 34 (IL34) expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 35 (IL35)

4-APC008Hu01
  • EUR 657.60
  • EUR 300.00
  • EUR 2136.00
  • EUR 792.00
  • EUR 1464.00
  • EUR 516.00
  • EUR 5160.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 35 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 29 (IL29)

4-APC029Hu01
  • EUR 1010.27
  • EUR 393.60
  • EUR 3458.50
  • EUR 1232.83
  • EUR 2345.66
  • EUR 751.20
  • EUR 8466.24
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 29 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 22 (IL22)

4-APC032Hu01
  • EUR 990.91
  • EUR 388.80
  • EUR 3385.92
  • EUR 1208.64
  • EUR 2297.28
  • EUR 739.20
  • EUR 8284.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 22 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 20 (IL20)

4-APC058Hu01
  • EUR 711.36
  • EUR 314.40
  • EUR 2337.60
  • EUR 859.20
  • EUR 1598.40
  • EUR 552.00
  • EUR 5664.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 20 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 24 (IL24)

4-APC064Hu01
  • EUR 711.36
  • EUR 314.40
  • EUR 2337.60
  • EUR 859.20
  • EUR 1598.40
  • EUR 552.00
  • EUR 5664.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 24 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 10 (IL10)

RPU54838-100ug 100ug
EUR 1030.7

Active Interleukin 10 (IL10)

RPU54838-1mg 1mg
EUR 4569.5

Active Interleukin 10 (IL10)

RPU54838-50ug 50ug
EUR 828.3

Active Interleukin 11 (IL11)

RPU54855-100ug 100ug
EUR 1108.8

Active Interleukin 11 (IL11)

RPU54855-1mg 1mg
EUR 4914

Active Interleukin 11 (IL11)

RPU54855-50ug 50ug
EUR 891

Active Interleukin 15 (IL15)

RPU54856-100ug 100ug
EUR 1232

Active Interleukin 15 (IL15)

RPU54856-1mg 1mg
EUR 6240

Active Interleukin 15 (IL15)

RPU54856-50ug 50ug
EUR 990

Active Interleukin 16 (IL16)

RPU54857-100ug 100ug
EUR 1108.8

Active Interleukin 16 (IL16)

RPU54857-1mg 1mg
EUR 4914

Active Interleukin 16 (IL16)

RPU54857-50ug 50ug
EUR 891

Active Interleukin 17 (IL17)

RPU54858-100ug 100ug
EUR 514.8

Active Interleukin 17 (IL17)

RPU54858-1mg 1mg
EUR 2607.8

Active Interleukin 17 (IL17)

RPU54858-50ug 50ug
EUR 401.3

Active Interleukin 18 (IL18)

RPU54859-100ug 100ug
EUR 722.7

Active Interleukin 18 (IL18)

RPU54859-1mg 1mg
EUR 3199.3

Active Interleukin 18 (IL18)

RPU54859-50ug 50ug
EUR 579.7

Active Interleukin 25 (IL25)

RPU54937-100ug 100ug
EUR 739.2

Active Interleukin 25 (IL25)

RPU54937-1mg 1mg
EUR 3276

Active Interleukin 25 (IL25)

RPU54937-50ug 50ug
EUR 594

Active Interleukin 35 (IL35)

RPU54947-100ug 100ug
EUR 616

Active Interleukin 35 (IL35)

RPU54947-1mg 1mg
EUR 2730

Active Interleukin 35 (IL35)

RPU54947-50ug 50ug
EUR 495

Active Interleukin 20 (IL20)

RPU54948-100ug 100ug
EUR 675.4

Active Interleukin 20 (IL20)

RPU54948-1mg 1mg
EUR 3419

Active Interleukin 20 (IL20)

RPU54948-50ug 50ug
EUR 526.1

Active Interleukin 24 (IL24)

RPU54949-100ug 100ug
EUR 651.2

Active Interleukin 24 (IL24)

RPU54949-1mg 1mg
EUR 3294.2

Active Interleukin 24 (IL24)

RPU54949-50ug 50ug
EUR 506.9

Active Interleukin 18 (IL18)

RPU55467-100ug 100ug
EUR 739.2

Active Interleukin 18 (IL18)

RPU55467-1mg 1mg
EUR 3731

Active Interleukin 18 (IL18)

RPU55467-50ug 50ug
EUR 574.1

Active Interleukin 21 (IL21)

RPU55704-100ug 100ug
EUR 997.7

Active Interleukin 21 (IL21)

RPU55704-1mg 1mg
EUR 3893.5

Active Interleukin 21 (IL21)

RPU55704-50ug 50ug
EUR 599.1

Active Interleukin 33 (IL33)

RPU55739-100ug 100ug
EUR 1013.1

Active Interleukin 33 (IL33)

RPU55739-1mg 1mg
EUR 5116.8

Active Interleukin 33 (IL33)

RPU55739-50ug 50ug
EUR 787.2

Active Interleukin 34 (IL34)

RPU55743-100ug 100ug
EUR 997.7

Active Interleukin 34 (IL34)

RPU55743-1mg 1mg
EUR 3931.2

Active Interleukin 34 (IL34)

RPU55743-50ug 50ug
EUR 604.8

Active Interleukin 29 (IL29)

RPU55746-100ug 100ug
EUR 651.2

Active Interleukin 29 (IL29)

RPU55746-1mg 1mg
EUR 3294.2

Active Interleukin 29 (IL29)

RPU55746-50ug 50ug
EUR 506.9

Active Interleukin 22 (IL22)

RPU55748-100ug 100ug
EUR 1282.6

Active Interleukin 22 (IL22)

RPU55748-1mg 1mg
EUR 5054.4

Active Interleukin 22 (IL22)

RPU55748-50ug 50ug
EUR 777.6

Active Interleukin 32 (IL32)

RPU58896-100ug 100ug
EUR 709.8

Active Interleukin 32 (IL32)

RPU58896-1mg 1mg
EUR 3294.7

Active Interleukin 32 (IL32)

RPU58896-50ug 50ug
EUR 522.7

Active Interleukin 19 (IL19)

RPU58930-100ug 100ug
EUR 868.2

Active Interleukin 19 (IL19)

RPU58930-1mg 1mg
EUR 3419.5

Active Interleukin 19 (IL19)

RPU58930-50ug 50ug
EUR 542.5

Active Interleukin 10 (IL10)

RPU59057-100ug 100ug
EUR 939.7

Active Interleukin 10 (IL10)

RPU59057-1mg 1mg
EUR 3673.8

Active Interleukin 10 (IL10)

RPU59057-50ug 50ug
EUR 587.2

Active Interleukin 31 (IL31)

RPU59177-100ug 100ug
EUR 1084.2

Active Interleukin 31 (IL31)

RPU59177-1mg 1mg
EUR 4504.5

Active Interleukin 31 (IL31)

RPU59177-50ug 50ug
EUR 720

Active Interleukin 22 (IL22)

RPU59213-100ug 100ug
EUR 1069.2

Active Interleukin 22 (IL22)

RPU59213-1mg 1mg
EUR 4738.5

Active Interleukin 22 (IL22)

RPU59213-50ug 50ug
EUR 712.8

Active Interleukin 10 (IL10)

RPU59216-100ug 100ug
EUR 1082.4

Active Interleukin 10 (IL10)

RPU59216-1mg 1mg
EUR 4797

Active Interleukin 10 (IL10)

RPU59216-50ug 50ug
EUR 721.6

Active Interleukin 11 (IL11)

RPU59219-100ug 100ug
EUR 1084.1

Active Interleukin 11 (IL11)

RPU59219-1mg 1mg
EUR 4802.9

Active Interleukin 11 (IL11)

RPU59219-50ug 50ug
EUR 722.5

Active Interleukin 26 (IL26)

RPU59230-100ug 100ug
EUR 1093.1

Active Interleukin 26 (IL26)

RPU59230-1mg 1mg
EUR 4843.8

Active Interleukin 26 (IL26)

RPU59230-50ug 50ug
EUR 728.6

Active Interleukin 10 (IL10)

RPU56353-100ug 100ug
EUR 1282.6

Active Interleukin 10 (IL10)

RPU56353-1mg 1mg
EUR 5054.4

Active Interleukin 10 (IL10)

RPU56353-50ug 50ug
EUR 777.6

Active Interleukin 10 (IL10)

RPU56368-100ug 100ug
EUR 1078

Active Interleukin 10 (IL10)

RPU56368-1mg 1mg
EUR 4777.5

Active Interleukin 10 (IL10)

RPU56368-50ug 50ug
EUR 866.8

Active Interleukin 2 (IL2)

4-APA073Hu61
  • EUR 872.64
  • EUR 357.60
  • EUR 2942.40
  • EUR 1060.80
  • EUR 2001.60
  • EUR 660.00
  • EUR 7176.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 2 expressed in: 293F cell

Active Interleukin 2 (IL2)

4-APA073Mu61
  • EUR 808.13
  • EUR 339.60
  • EUR 2700.48
  • EUR 980.16
  • EUR 1840.32
  • EUR 616.80
  • EUR 6571.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Interleukin 2 expressed in: 293F cell

Active Interleukin 4 (IL4)

4-APA077Hu01
  • EUR 798.46
  • EUR 337.20
  • EUR 2664.19
  • EUR 968.06
  • EUR 1816.13
  • EUR 609.60
  • EUR 6480.48
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 4 expressed in: E.coli

Active Interleukin 4 (IL4)

4-APA077Hu61
  • EUR 939.30
  • EUR 375.60
  • EUR 3192.38
  • EUR 1144.13
  • EUR 2168.26
  • EUR 704.40
  • EUR 7800.96
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 4 expressed in: 293F cell

Active Interleukin 4 (IL4)

4-APA077Mu01
  • EUR 781.25
  • EUR 332.40
  • EUR 2599.68
  • EUR 946.56
  • EUR 1773.12
  • EUR 598.80
  • EUR 6319.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Interleukin 4 expressed in: E.coli

Active Interleukin 4 (IL4)

4-APA077Ra01
  • EUR 995.21
  • EUR 390.00
  • EUR 3402.05
  • EUR 1214.02
  • EUR 2308.03
  • EUR 741.60
  • EUR 8325.12
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Interleukin 4 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 6 (IL6)

4-APA079Hu01
  • EUR 872.64
  • EUR 357.60
  • EUR 2942.40
  • EUR 1060.80
  • EUR 2001.60
  • EUR 660.00
  • EUR 7176.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 6 expressed in: E.coli

Active Interleukin 6 (IL6)

4-APA079Hu61
  • EUR 968.33
  • EUR 382.80
  • EUR 3301.25
  • EUR 1180.42
  • EUR 2240.83
  • EUR 723.60
  • EUR 8073.12
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 6 expressed in: 293F cell

Active Interleukin 6 (IL6)

4-APA079Mu61
  • EUR 942.53
  • EUR 375.60
  • EUR 3204.48
  • EUR 1148.16
  • EUR 2176.32
  • EUR 706.80
  • EUR 7831.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Interleukin 6 expressed in: 293F cell

Active Interleukin 6 (IL6)

4-APA079Ra61
  • EUR 872.64
  • EUR 357.60
  • EUR 2942.40
  • EUR 1060.80
  • EUR 2001.60
  • EUR 660.00
  • EUR 7176.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Interleukin 6 expressed in: 293F cell

Active Interleukin 8 (IL8)

4-APA080Hu01
  • EUR 700.61
  • EUR 310.80
  • EUR 2297.28
  • EUR 845.76
  • EUR 1571.52
  • EUR 544.80
  • EUR 5563.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 8 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 7 (IL7)

4-APA662Hu01
  • EUR 993.06
  • EUR 390.00
  • EUR 3393.98
  • EUR 1211.33
  • EUR 2302.66
  • EUR 740.40
  • EUR 8304.96
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 7 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 4 (IL4)

RPU54749-100ug 100ug
EUR 777.7

Active Interleukin 4 (IL4)

RPU54749-1mg 1mg
EUR 3445

Active Interleukin 4 (IL4)

RPU54749-50ug 50ug
EUR 624.8

Active Interleukin 2 (IL2)

RPU54751-100ug 100ug
EUR 453.2

Active Interleukin 2 (IL2)

RPU54751-1mg 1mg
EUR 2009.8

Active Interleukin 2 (IL2)

RPU54751-50ug 50ug
EUR 364.1

Active Interleukin 4 (IL4)

RPU54828-100ug 100ug
EUR 822.8

Active Interleukin 4 (IL4)

RPU54828-1mg 1mg
EUR 3244.8

Active Interleukin 4 (IL4)

RPU54828-50ug 50ug
EUR 499.2

Active Interleukin 6 (IL6)

RPU54829-100ug 100ug
EUR 1045

Active Interleukin 6 (IL6)

RPU54829-1mg 1mg
EUR 5253.3

Active Interleukin 6 (IL6)

RPU54829-50ug 50ug
EUR 808.4

Active Interleukin 6 (IL6)

RPU54832-100ug 100ug
EUR 971.3

The purpose of this study to analyze the content of inflammatory cytokines in the fracture hematoma Human and determine their effect on osteoprogenitor cells. Twenty-three patients were recruited following informed consent. peripheral blood, and bone fracture hematoma collected. A Luminex assay at the level of 34 cytokines done and autologous peripheral blood samples served as controls. Mesenchymal Stem Cells (MSCs) were isolated following collagenase digestion and functional tests performed. Gene expression analysis of 84 key osteogenic molecules do.

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