Deoxynivalenol (DON) is an unavoidable contaminants in the Human of food, fodder and agricultural products. Growth retardation in children caused by pollution comprehensive DON has become a global problem that can not be ignored. Previous research has shown that DON causes stunting in children with bowel dysfunction, insulin-like growth factor-1 (IGF-1) axis disruption and peptide YY (PYY). Galanin-like peptide (GALP) is a growth regulator that is important, but its role in DON-induced growth retardation is unclear.
In this study, we report the important role GALP for DON-induced growth inhibition in rat pituitary tumor cell line GH3. DON was found to increase the expression of GALP through hypomethylationin promoter region of GALP gene and upregulate the expression of proinflammatory factors, while downregulate the expression of growth hormone (GH). Furthermore, GALP promoted excessive proinflammatory cytokines, including TNF-α, IL -1β, IL – 11 and IL -6, and will decrease cell viability and cell proliferation, while the inhibitory effect of GALP is the opposite.
GALP expression and insulin-like growth factor binding protein acidic labile subunit (IGFALS) showed the opposite trend, which is the reason the potential regulation of cell proliferation by GALP. Additionally, GALP has anti-apoptotic effects, which can eliminate the inflammatory damage to the cells, thereby contributing to the inhibition of cell growth.
These findings provide new mechanistic insight into the toxicity of DON-induced growth retardation and suggest the therapeutic potential GALP on DON-related diseases.
Epigenetic upregulation of galanin-like peptide mediates deoxynivalenol induced-growth inhibition in pituitary cells
The immunoregulatory and neuroprotective effects of Human of adipose derived stem cells that express the IL – 11 and IL in the experimental -13 autoimmune encephalomyelitis mice
Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS) characterized by incomplete endogenous remyelination in the chronic phase. The shift of cytokine balance between pro and anti-inflammatory is one important marker in the patho gene is from MS. This study aimed to evaluate the effect of Human of adipose-derived stem cells (hADSCs) expressing interleukin 11 and interleukin 13 ( IL – 11 , 13-hADSCs) in experimental autoimmune encephalomyelitis (EAE), an animal model of MS.
12 days after immunization C57BL / 6 female mice with MOG35-55 and the early appearance of clinical symptoms, IL – < em> 11 , 13-hADSCs injected via the tail vein into mice EAE. Then, the mice were sacrificed at 30 days post-immunization (DPI) and the spinal cord of the experimental group were extracted for histopathological and real-time RT-PCR results showed that clinical scores studies.
The and infiltration of mononuclear cells into the spinal cords of EAE mice was significantly reduced in mice treated with IL – 11 , 13-hADSCs. Similarly, remyelination and oligodendro gene was significantly improved in the treatment group mentioned. Real-time results show that the cytokine pro / anti-inflammatory gene expression overturned in the IL – 11 , 13-hADSCs treatment group compared to those without EAE untreated group.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Rat Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Rat Interleukin 20 (IL20)serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 20 (IL20) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Interleukin 20 (IL20) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Interleukin 20 (IL20) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interleukin 20 (IL20) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interleukin 20 (IL20) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interleukin 20 (IL20) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Interleukin 20 (IL20) in serum, plasma and other biological fluids.
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Interleukin 20 (IL20)Serum, plasma and other biological fluids
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 20 (IL20) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Interleukin 20 (IL20) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 20 (IL20) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Interleukin 20 (IL20) in samples from serum, plasma or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Interleukin 20 (IL20) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Interleukin 20 (IL20) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 20 (IL20) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 20 (IL20) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 20 (IL20) in serum, plasma and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interleukin 20 (IL20) in serum, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Interleukin 20 (IL20) in samples from Serum, plasma and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: Quantitativesandwich ELISA kit for measuring Mouse Interleukin-20 (IL20) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Interleukin-20(IL20) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Expression of IL – 11 as the neurotrophic cytokine and IL -13 as an anti-inflammatory cytokine with hADSCs can improve immunomodulatory and neuroprotective effects of hADSCs and a strong candidate in stem cell therapies for the future treatment of MS.
