Is the seminal plasma (SP) and its constituents affect decidualizing capacity and transcriptome of Human main endometrial stromal fibroblasts (eSFs)? SP promoting decidualizing of eSFs of women with and without inflammatory disorders (polycystic ovary syndrome (PCOS), endometriosis) in a way that is not mediated by amyloid semen and those associated with the response transcription powerful, including the induction of interleukin ( IL ) – 11 , cytokines important for the SP-induced decidualizing.

Clinical studies have shown that SP may promote implantation, and in vitro studies have shown that SP can promote decidualizing, steroid hormone driven program differentiation of the ESF which is essential for embryo implantation and impaired in women with disorders of PCOS inflammatory and endometriosis. This is a cross-sectional study in volving samples treated with vehicle alone compared to treatment with SP or SP constituents. SP was tested for the ability to promote decidualizing in vitro in eSFs of women with or without PCOS or endometriosis (n = 9). The role of amyloid semen and SP fractionated in mediating these effects and in eliciting changes in transcription in eSFs were studied.

Finally, the role of IL -. 11 , cytokines with a key role in the implantation and decidualizing, rated as a mediator of decidualizing SP-facilitated eSFs and endometrial epithelial cells (EECS) was isolated from a biopsy of the endometrium of women of reproductive age undergoing benign gynecological procedure and maintained in vitro. Tests were performed to assess whether treatment with SP or SP eSFs constituents affect the rate and extent of decidualizing in women with and without inflammatory disorders.

To characterize the response of the endometrium to SP and SP constituents, RNA was isolated from treated or EECS eSFs and analyzed by RNA sequencing (RNAseq). Factors secreted from cells treated conditioned media were analyzed by Luminex and ELISA. Role IL -. 11 in the SP-induced decidualizing assessed through Clustered interspaced regularly repeated palindromic short (CRISPR) / Cas-9-mediated knockout experiments in eSFs primary SP promoted decidualizing both in the absence and presence steroid hormone (P <0.05 compared to vehicle) with the necessary means seminal proteins.

amyloid semen does not promote decidualizing and induced a weak and transcriptomic responses in eSFs secretomic. Instead, fractionated SP enriched for semen microvesicles (MV) decidualizing promoted. IL – 11 is one of the most potent SP-induced gene in eSFs and important for decidualizing SP-facilitated <. Data AbstractText> RNAseq deposited in Gene Expression Omnibus repository with the serial number GSE135640 accession. This study is limited to in vitro analysis. Our results support the idea that the SP promotes decidualizing, including in eSFs of women with inflammatory disorders.

Active Interleukin 16 (IL16)
MBS2097241-001mg	MyBiosource	0.01mg	EUR 265

Active Interleukin 16 (IL16)
MBS2097241-005mg	MyBiosource	0.05mg	EUR 575

Active Interleukin 16 (IL16)
MBS2097241-01mg	MyBiosource	0.1mg	EUR 885

Active Interleukin 16 (IL16)
MBS2097241-02mg	MyBiosource	0.2mg	EUR 1090

Active Interleukin 16 (IL16)
MBS2097241-05mg	MyBiosource	0.5mg	EUR 2130

Active Interleukin 16 (IL16)
RPU54857-100ug	Biomatik Corporation	100ug	EUR 1108.8

Active Interleukin 16 (IL16)
RPU54857-1mg	Biomatik Corporation	1mg	EUR 4914

Active Interleukin 16 (IL16)
RPU54857-50ug	Biomatik Corporation	50ug	EUR 891

Human Interleukin 16 (IL16) Protein (Active)
abx651376-100g	Abbexa	100 µg	EUR 1062.5

Human Interleukin 16 (IL16) Protein (Active)
20-abx651376	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	10 ug 50 ug 100 ug 200 ug 1 mg

Human Interleukin 16 (IL16) Protein (Active)
abx651376-200g	Abbexa	200 µg	EUR 1300

Human Interleukin 16 (IL16) Protein (Active)
abx651376-50g	Abbexa	50 µg	EUR 700

Recombinant Human interleukin-16 proein (IL16) (Active)
CSB-AP001641HU	Cusabio	8250 mg	Ask for price

Recombinant Human interleukin-16 proein (IL16) (Active)
CSB-AP001641HU-100ug	Cusabio	100ug	Ask for price
Description: 1212-1332aa

Recombinant Human interleukin-16 proein (IL16) (Active)
CSB-AP001641HU-10ug	Cusabio	10ug	Ask for price
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Recombinant Human interleukin-16 proein (IL16) (Active)
CSB-AP001641HU-500ug	Cusabio	500ug	Ask for price
Description: 1212-1332aa

Recombinant Human interleukin-16 proein(IL16) (Active)
AP73454	SAB	1mg	EUR 6829

Recombinant Human interleukin-16 proein (IL16) (Active)
MBS969282-001mg	MyBiosource	0.01mg	EUR 355

Recombinant Human interleukin-16 proein (IL16) (Active)
MBS969282-01mg	MyBiosource	0.1mg	EUR 1590

Recombinant Human interleukin-16 proein (IL16) (Active)
MBS969282-05mg	MyBiosource	0.5mg	EUR 3395

Recombinant Human interleukin-16 proein (IL16) (Active)
MBS969282-5x05mg	MyBiosource	5x0.5mg	EUR 15240

Recombinant Human interleukin-16 protein(IL16) (Active)
AP73453	SAB	1mg	EUR 6829

Recombinant Human interleukin-16 protein (IL16) (Active)
MBS969281-001mg	MyBiosource	0.01mg	EUR 355

Recombinant Human interleukin-16 protein (IL16) (Active)
MBS969281-01mg	MyBiosource	0.1mg	EUR 1590

Recombinant Human interleukin-16 protein (IL16) (Active)
MBS969281-05mg	MyBiosource	0.5mg	EUR 3395

Recombinant Human interleukin-16 protein (IL16) (Active)
MBS969281-5x05mg	MyBiosource	5x0.5mg	EUR 15240

Recombinant Human interleukin-16 protein (IL16), partial (Active)
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Recombinant Human interleukin-16 protein (IL16), partial (Active)
CSB-AP001631HU-100ug	Cusabio	100ug	Ask for price
Description: 1203-1332aa

Recombinant Human interleukin-16 protein (IL16), partial (Active)
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Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16) (Active)
CSB-AP003381MO	Cusabio	8303 mg	Ask for price

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Description: 1205-1322aa

Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein(Il16) (Active)
AP73456	SAB	1mg	EUR 8192

Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16) (Active)
MBS969456-001mg	MyBiosource	0.01mg	EUR 355

Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16) (Active)
MBS969456-01mg	MyBiosource	0.1mg	EUR 1900

Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16) (Active)
MBS969456-05mg	MyBiosource	0.5mg	EUR 4065

Recombinant Mouse Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (Il16) (Active)
MBS969456-5x05mg	MyBiosource	5x0.5mg	EUR 18245

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CSB-AP003191MOW	Cusabio	8289 mg	Ask for price

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Description: 510-630aa

Recombinant Rhesus Macaque Pro-interleukin-16 [Cleaved into: Interleukin-16 protein(IL16) (Active)
AP73455	SAB	1mg	EUR 8192

Recombinant Rhesus Macaque Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (IL16) (Active)
MBS969437-001mg	MyBiosource	0.01mg	EUR 355

Recombinant Rhesus Macaque Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (IL16) (Active)
MBS969437-01mg	MyBiosource	0.1mg	EUR 1900

Recombinant Rhesus Macaque Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (IL16) (Active)
MBS969437-05mg	MyBiosource	0.5mg	EUR 4065

Recombinant Rhesus Macaque Pro-interleukin-16 [Cleaved into: Interleukin-16 protein (IL16) (Active)
MBS969437-5x05mg	MyBiosource	5x0.5mg	EUR 18245

Active Interleukin 10 (IL10)
APA056Bo61	Cloud-Clone	10ug	EUR 368

Active Interleukin 10 (IL10)
APA056Ca61	Cloud-Clone	10ug	EUR 328

Active Interleukin 10 (IL10)
APA056Eq61	Cloud-Clone	10ug	EUR 380

Active Interleukin 10 (IL10)
APA056Ga61	Cloud-Clone	10ug	EUR 319

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APA056Hu61	Cloud-Clone	10ug	EUR 335

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4-APA056Hu61	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	10ug 50ug 100 ug 200 ug 500 ug 1 mg 5 mg
Description: Recombinant Human Interleukin 10 expressed in: 293F cell

Active Interleukin 10 (IL10)
APA056Mu61	Cloud-Clone	10ug	EUR 324

Active Interleukin 10 (IL10)
APA056Ra01	Cloud-Clone	10ug	EUR 350

Active Interleukin 10 (IL10)
APA056Ra61	Cloud-Clone	10ug	EUR 251

Active Interleukin 10 (IL10)
APA056Rb61	Cloud-Clone	10ug	EUR 331

Active Interleukin 11 (IL11)
APA057Hu01	Cloud-Clone	10ug	EUR 360

Active Interleukin 11 (IL11)
4-APA057Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	10ug 50ug 100 ug 200 ug 500 ug 1 mg 5 mg
Description: Recombinant Human Interleukin 11 expressed in: E.coli

Active Interleukin 11 (IL11)
APA057Hu61	Cloud-Clone	10ug	EUR 328

Active Interleukin 11 (IL11)
APA057Hu62	Cloud-Clone	10ug	EUR 332

Active Interleukin 13 (IL13)
APA060Hu01	Cloud-Clone	10ug	EUR 317

Active Interleukin 13 (IL13)
APA060Ra01	Cloud-Clone	10ug	EUR 211

Active Interleukin 13 (IL13)
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Active Interleukin 15 (IL15)
APA061Hu01	Cloud-Clone	10ug	EUR 400

Active Interleukin 15 (IL15)
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Active Interleukin 17 (IL17)
APA063Hu01	Cloud-Clone	10ug	EUR 167

Active Interleukin 17 (IL17)
4-APA063Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	10ug 50ug 100 ug 200 ug 500 ug 1 mg 5 mg
Description: Recombinant Human Interleukin 17 expressed in: E.coli

Active Interleukin 17 (IL17)
APA063Mu61	Cloud-Clone	10ug	EUR 272

Active Interleukin 17 (IL17)
APA063Po61	Cloud-Clone	10ug	EUR 280

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APA064Hu01	Cloud-Clone	10ug	EUR 239

Active Interleukin 18 (IL18)
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Description: Recombinant Human Interleukin 18 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 18 (IL18)
APA064Mu01	Cloud-Clone	10ug	EUR 233

Active Interleukin 18 (IL18)
4-APA064Mu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	10ug 50ug 100 ug 200 ug 500 ug 1 mg 5 mg
Description: Recombinant Mouse Interleukin 18 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 19 (IL19)
APB190Hu61	Cloud-Clone	10ug	EUR 350

Active Interleukin 19 (IL19)
APB190Mu61	Cloud-Clone	10ug	EUR 380

Active Interleukin 19 (IL19)
APB190Ra61	Cloud-Clone	10ug	EUR 372

Active Interleukin 21 (IL21)
APB688Hu01	Cloud-Clone	10ug	EUR 250

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4-APB688Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	5 mg 10ug 50ug 100 ug 200 ug 500 ug 1 mg
Description: Recombinant Human Interleukin 21 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 21 (IL21)
APB688Hu61	Cloud-Clone	10ug	EUR 300

Active Interleukin 21 (IL21)
APB688Ra61	Cloud-Clone	10ug	EUR 269

Active Interleukin 25 (IL25)
APB694Hu01	Cloud-Clone	10ug	EUR 240

Active Interleukin 25 (IL25)
4-APB694Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	5 mg 10ug 50ug 100 ug 200 ug 500 ug 1 mg
Description: Recombinant Human Interleukin 25 expressed in: E.coli

Active Interleukin 25 (IL25)
APB694Hu61	Cloud-Clone	10ug	EUR 280

Active Interleukin 25 (IL25)
APB694Ra61	Cloud-Clone	10ug	EUR 272

Active Interleukin 33 (IL33)
APB980Hu01	Cloud-Clone	10ug	EUR 328

Active Interleukin 33 (IL33)
4-APB980Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	5 mg 10ug 50ug 100 ug 200 ug 500 ug 1 mg
Description: Recombinant Human Interleukin 33 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 34 (IL34)
APC007Hu01	Cloud-Clone	10ug	EUR 252

Active Interleukin 34 (IL34)
4-APC007Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	5 mg 10ug 50ug 100 ug 200 ug 500 ug 1 mg
Description: Recombinant Human Active Interleukin 34 (IL34) expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 34 (IL34)
APC007Hu61	Cloud-Clone	10ug	EUR 316

Active Interleukin 34 (IL34)
APC007Mu01	Cloud-Clone	10ug	EUR 326

Active Interleukin 34 (IL34)
APC007Mu61	Cloud-Clone	10ug	EUR 320

Active Interleukin 35 (IL35)
APC008Hu01	Cloud-Clone	10ug	EUR 200

Active Interleukin 35 (IL35)
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Description: Recombinant Human Interleukin 35 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 29 (IL29)
APC029Hu01	Cloud-Clone	10ug	EUR 211

Active Interleukin 29 (IL29)
4-APC029Hu01	Cloud-Clone	Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price Ask for price	5 mg 10ug 50ug 100 ug 200 ug 500 ug 1 mg
Description: Recombinant Human Interleukin 29 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 29 (IL29)
APC029Ra61	Cloud-Clone	10ug	EUR 280

Active Interleukin 22 (IL22)
APC032Hu01	Cloud-Clone	10ug	EUR 324

Active Interleukin 22 (IL22)
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Description: Recombinant Human Interleukin 22 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 22 (IL22)
APC032Hu61	Cloud-Clone	10ug	EUR 324

Active Interleukin 22 (IL22)
APC032Mu61	Cloud-Clone	10ug	EUR 276

Active Interleukin 20 (IL20)
APC058Hu01	Cloud-Clone	10ug	EUR 219

Active Interleukin 20 (IL20)
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Description: Recombinant Human Interleukin 20 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 20 (IL20)
APC058Mu61	Cloud-Clone	10ug	EUR 371

Active Interleukin 24 (IL24)
APC064Hu01	Cloud-Clone	10ug	EUR 211

Active Interleukin 24 (IL24)
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Description: Recombinant Human Interleukin 24 expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Active Interleukin 23 (IL23)
APX262Mu61	Cloud-Clone	10ug	EUR 340

Active Interleukin 10 (IL10)
MBS2086062-001mg	MyBiosource	0.01mg	EUR 255

Active Interleukin 10 (IL10)
MBS2086062-005mg	MyBiosource	0.05mg	EUR 535

Active Interleukin 10 (IL10)
MBS2086062-01mg	MyBiosource	0.1mg	EUR 825

Active Interleukin 10 (IL10)
MBS2086062-02mg	MyBiosource	0.2mg	EUR 1020

Active Interleukin 10 (IL10)
MBS2086062-05mg	MyBiosource	0.5mg	EUR 1985

Active Interleukin 13 (IL13)
MBS2105067-001mg	MyBiosource	0.01mg	EUR 190

Active Interleukin 13 (IL13)
MBS2105067-005mg	MyBiosource	0.05mg	EUR 355

Active Interleukin 13 (IL13)
MBS2105067-01mg	MyBiosource	0.1mg	EUR 540

Active Interleukin 13 (IL13)
MBS2105067-02mg	MyBiosource	0.2mg	EUR 660

Active Interleukin 13 (IL13)
MBS2105067-05mg	MyBiosource	0.5mg	EUR 1270

Active Interleukin 18 (IL18)
MBS2105068-001mg	MyBiosource	0.01mg	EUR 205

Active Interleukin 18 (IL18)
MBS2105068-005mg	MyBiosource	0.05mg	EUR 400

Active Interleukin 18 (IL18)
MBS2105068-01mg	MyBiosource	0.1mg	EUR 610

Active Interleukin 18 (IL18)
MBS2105068-02mg	MyBiosource	0.2mg	EUR 745

Despite the general ability of amyloid to induce cytokines known to be important for implantation, semen amyloid bad marks to decidualizing eSFs and do not promote them. In contrast, SP fractionated enriched for MV promoted transcription decidualizing and triggers a response in eSFs overlapping with the SP.